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. 2009 Sep 11;8(11):1732–1738. doi: 10.1128/EC.00135-09

FIG. 8.

FIG. 8.

Electrophoretic karyotypes of parental and hybrid strains of A. alternata. Chromosome-size DNA was separated by PFGE under conditions for <2.0 Mb DNA (a, b, and c) and for 1.0 to 6.0 Mb DNA (d). The blots were probed with AFT1 (b) from the strawberry pathotype and ALT1 (c) from the tomato pathotype. Chromosome-size DNA was separated in a 0.8% agarose gel run at 5.5 V/cm with pulse intervals of 120 s for 12 h and 180 s for 12 h (a, b, and c) and at 1.5 V/cm for 48 h (d) with pulse intervals of 1,500 to 3,000 s, at 1.8 V/cm for 40 h with 900 to 1,500 s, and 2.4 V/cm for 57 h with 480 to 900 s. 27G-1, Geneticin-resistant parental strain derived from the A. alternata tomato pathotype (As-27) harboring an npt gene; TP1, hygromycin B-resistant parental strain derived from the A. alternata strawberry pathotype (NAF8) harboring an hph gene; EST6, the fusion strain between 27G-1 and TP1.