FIG. 1.

Zymocin hypersensitivity of TOR-defective tap42^ts mutants. (A) Zymocin profiles. The indicated S. cerevisiae strains (wt, wild type) were serially diluted and replica spotted onto YPD medium lacking (− zymo) or containing (+ zymo) 40% or 50% (vol/vol) zymocin. To assay the impact of SSD1-v on thermosensitivity and zymocin action, the same strains carrying (SSD1-v) or lacking (vector) an extra copy of SSD1-v were grown for 3 days at the temperatures indicated. Thermosensitive (ts), as well as zymocin-resistant (R), -sensitive (S), or -hypersensitive (HS), traits are indicated. (B) SSD1-v suppresses heat-sensitive Sit4/Tap42 dissociation in tap42-11^ts cells. Sit4-HA-expressing tap42-11^ts cells with (SSD1-v) or without (vector) an extra SSD1-v copy were cultivated at the indicated temperatures and subjected to immune precipitation (IP) using anti-HA antibodies. The presence of Sit4 and Tap42-11 (indicated by arrows) in these precipitates was monitored by immune blotting (IB) using anti-HA and anti-Tap42 antibodies, respectively. In addition, prior to immune precipitation (preIP), the content of Sit4 and Tap42-11 in the input was checked by Western blotting.