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Complementation of AVO1 deletion with the rmsA gene in S. cerevisiae. Cell suspensions of S. cerevisiae strains were spotted onto solid YP-glucose or YP-galactose medium and inoculated for 3 days at 28°C. Cells were spotted in a 10-fold dilution series starting with ∼1 × 105 cells as the highest concentration. Note that the GAL1 promoter allows leaky expression under noninduced conditions (glucose). wt, wild type.
