FIG. 5.

Changes in plasma membrane phosphatidylserine distribution during apoptosis induced either by H₂O₂ (1 mM) or CPT (5 μM). Control (CT) and LmAPX-overexpressing (OE) cells were double stained with annexin V and PI and analyzed by flow cytometric analysis at the indicated times. Representative dot plots are divided in four quadrants. Viable cells that did not bind annexin V and also did not incorporate PI are represented by the lower left quadrant of each dot plot, early apoptotic cells that bind only annexin V are represented by the lower right quadrant, late apoptotic cells that bind annexin V and incorporate PI are represented by the upper right quadrant. Percentages of cells are indicated in the corresponding quadrants. (A) Time-dependent distribution of viable, early apoptotic, and late apoptotic cells among H₂O₂-challenged control cells (upper panel) and LmAPX-overexpressing cells (lower panel). (B) Time-dependent distribution of viable, early apoptotic, and late apoptotic cells among CPT-treated control cells (upper panel) and LmAPX-overexpressing cells (lower panel). A total of 30,000 cells were analyzed in each condition. All results are representative of three independent experiments.