TABLE 2.
Depletion of drug-induced caspase-like protease activity by LmAPX
| Cells | FITC green fluorescence (mean ± SEM)a with: |
|||||
|---|---|---|---|---|---|---|
| No FLICA | FLICA | FLICA + H2O2 | FLICA + CPT | H2O2 + VAD-fmk + FLICA | CPT + VAD-fmk + FLICA | |
| Control | 126 ± 5 | 278 ± 10 | 434 ± 18* | 397 ± 30* | 328 ± 20* | 307 ± 22 |
| LmAPX overexpressing | 142 ± 8 | 258 ± 9 | 281 ± 7* | 283 ± 12* | 271 ± 10* | 277 ± 15 |
a
Increased FLICA green fluorescence indicates increased caspase-like protease activity. Unlabeled control cells were used to set up a flow cytometer by adjusting the forward and side scatter of the cell population. Values without FLICA indicate the background fluorescence level of unlabeled cells. The increase in fluorescence intensity was calculated with respect to background fluorescence intensity. The fluorescence data are representative of three independent experiments. Quantitation of the relative change in fluorescence was analyzed with FACSDiVa software. *, P < 0.05.