TABLE 1.
HPLC analysis of tocopherols and plastoquinonea
| Strain | Mean amt (pmol/106 cells) ± SD (%)b |
Mean plastoquinone level (nmol/g [dry weight]) ± SDc | ||||
|---|---|---|---|---|---|---|
| Total tocopherol | α-Tocopherol | β-Tocopherol | γ-Tocopherol | δ-Tocopherol | ||
| Wild type | 10.1 ± 1.7 | 8.2 ± 1.4 (81) | 0.4 ± 0.1 (4) | 1.0 ± 0.5 (10) | 0.5 ± 0.1 (5) | 294 ± 24 |
| vte3 mutant | 14.0 ± 1.9 | 0 (0) | 13.7 ± 1.8 (98) | 0 (0) | 0.3 ± 0.1 (2) | 318 ± 32 |
| Complemented vte3 mutant | 27.0 ± 5.5 | 25 ± 5.4 (92) | 0.2 ± 0.0 (1) | 1.7 ± 0.1 (6) | 0.1 ± 0.0 (1) | NA |
| npq1 lor1 mutant | 10.0 ± 0.2 | 9.0 ± 0.2 (90) | 0.3 ± 0.1 (4) | 0.5 ± 0.1 (4) | 0.2 ± 0.1 (2) | NA |
| vte3 npq1 lor1 mutant | 10.1 ± 1.0 | 0 (0) | 9.9 ± 1.0 (98) | 0 (0) | 0.2 ± 0.1 (2) | NA |
| VTE3/VTE3 diploid | 29.6 ± 4.3 | 24.3 ± 4.1 (82) | 2.7 ± 1.7 (9) | 2.0 ± 1.3 (7) | 0.6 ± 0.3 (2) | NA |
| vte3/vte3 diploid | 12.3 ± 3.4 | 0 (0) | 11.7 ± 3.1 (95) | 0 (0) | 0.6 ± 0.6 (5) | NA |
| VTE3/vte3 diploid | 36.5 ± 11.3 | 25.5 ± 5.8 (70) | 7.3 ± 4.5 (20) | 2.1 ± 1.5 (6) | 1.6 ± 1.2 (4) | NA |
a
The data are means ± SD (n = 3 to 6).
b
The percentage of each tocopherol in the total pool is given in parentheses.
c
NA, not applicable.