FIG. 3.

Growth profiles of the best candidate, 4s, harboring the nisin immunity genes expressed under the control of a synthetic promoter. All strains were grown in LB medium at 37°C; at an OD₆₀₀ of 0.5, cultures received four different nisin concentrations (600, 700, 800, and 900 ng/ml), and growth in the presence of nisin was compared to growth without the nisin addition. The black arrow indicates the time of the different nisin additions.