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. 2009 Sep 4;75(21):6947–6953. doi: 10.1128/AEM.00605-09

TABLE 1.

Typing of E. coli O26 isolates

Sampling day, source, and isolate Serotype Virulence profile by:
fliC PCR-RFLP stx1 gene stx2 gene Stx1 (toxin) Stx2 (toxin) Subtype(s)
 efa1 geneb EHEC-hlyA gene katP gene espP gene Plasmid size(s) in kb Cluster
stx1/stx2 eae tir espA espB
Day 15
    Animal 6 (farm A)
        WH-01/06/002-1 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
        WH-01/06/002-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
        WH-01/06/002-3 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
    Animal 8 (farm A)
        WH-01/08/002-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
    Animal 26 (farm A)
        WH-01/26/001-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12 7
        WH-01/26/001-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
        WH-01/26/001-6 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 7
        WH-01/26/001-7 O26:H11 H11 + − + − stx1 β β β β +/− + + + 110, 12 7
Day 29
    Animal 2 (farm A)
        WH-01/02/003-1 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 6
        WH-01/02/003-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 6
        WH-01/02/003-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 6
        WH-01/02/003-6 O26:H11 H11 + − + − stx1 β β β β +/+ − + + 110, 12 6
        WH-01/02/003-7 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 6
        WH-01/02/003-8 O26:H11 H11 + − + − stx1 β β β β −/+ + + + 110, 12 6
        WH-01/02/003-9 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110 6
        WH-01/02/003-10 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110 6
    Animal 26 (farm A)
        WH-01/26/002-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12 5
        WH-01/26/002-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12 5
        WH-01/26/002-8 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12 5
        WH-01/26/002-9 O26:H11 H11 + − + − stx1 β β β β +/+ + − − 110, 12 5
        WH-01/26/002-10 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12 5
Day 64
    Animal 20 (farm A)
        WH-01/20/005-3 O26:H11 H11 + − + − stx1 β β β β +/+ − − − 130, 2.5 2
Day 78
    Animal 29 (farm A)
        WH-01/29/002-1 O26:H11 H11 + − + − stx1 β β β β +/− + − − 130, 12, 2.5 4
        WH-01/29/002-2 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12, 2.5 4
        WH-01/29/002-3 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12, 2.5 4
        WH-01/29/002-4 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12, 2.5 4
        WH-01/29/002-5 O26:H11 H11 + − + − stx1 β β β β +/+ − − + 130, 12, 2.5 4
Day 106
    Animal 27 (farm A)
        WH-01/27/005-2 O26:H11 H11 + − + − stx1 β β β β +/− + + + 145, 110, 12 3
        WH-01/27/005-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 12, 2.5 5
        WH-01/27/005-6 O26:H11 H11 + − + − stx1 β β β β +/+ − − − 130, 12, 2.5 5
Day 113
    Animal 7 (farm C)
        WH-04/07/001-2 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ − + + 55, 35, 2.5 11
        WH-04/07/001-4 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 55 12
        WH-04/07/001-6 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 55 12
Day 170
    Animal 22 (farm C)
        WH-04/22/001-1 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12, 6.3 12
        WH-04/22/001-4 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12, 6.3 12
        WH-04/22/001-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12, 6.3 12
Day 176
    Animal 14 (farm D)
        WH-03/14/004-8 O26:H11 H11 + − + − stx1 β β β β +/+ − + + 110 10
Day 218
    Animal 27 (farm A)
        WH-01/27/009-1 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 9
        WH-01/27/009-2 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 9
        WH-01/27/009-3 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 8
        WH-01/27/009-8 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + − − 110, 12 8
        WH-01/27/009-9 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 9
Day 309
    Animal 29 (farm A)
        WH-01/29/010-1 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 35, 12 4
        WH-01/29/010-2 O26:H11 H11 + − + − stx1 β β β β +/+ + − − 130, 55, 35 8
        WH-01/29/010-3 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 130, 35, 12 8
Day 365
    Animal 8 (farm C)
        WH-04/08/008-6 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 55 12
Day 379
    Animal 9 (farm A)
        WH-01/09/016-2 O26:H32 H32 + + − − stx1/stx2 − − − − −/− − − − 145, 130, 1.8 1
    Animal 27 (farm A)
        WH-01/27/014-3 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 9
        WH-01/27/014-4 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 9
        WH-01/27/014-5 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12 8
Day 407
    Animal 29 (farm A)
        WH-01/29/013-4 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12, 2.5 8
        WH-01/29/013-7 O26:H11 H11 + − + − stx1 β β β β +/+ + + + 110, 12, 2.5 8
Day 478
    Animal 27 (farm A)
        WH-01/27/017-1 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 8
        WH-01/27/017-5 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110, 12 8
        WH-01/27/017-6 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110 8
        WH-01/27/017-7 O26:H11 H11 + + + + stx1/stx2 β β β β +/+ + + + 110 8
        WH-01/27/017-10 O26:H11 H11 + − + + stx1 β β β β +/+ + + + 130, 12, 2.5 8
a

stx1/stx2, gene stx1 or stx2.

b

efa1 was detected by two hybridizations (with lifA1-lifA2 and lifA3-lifA4 probes). +/+, complete gene; +/− or −/+, incomplete gene; −/−, efa1 negative.