Mean mcrA transcript and gene abundances during time course incubations at 25°C. Results are shown for slurries constructed from peat stored for 6 months (A) and 4 months (B, C). (A, B) mcrA abundances normalized to quantities ng−1 nucleic acid. (C) mcrA abundances g−1 soil (dry weight), corrected by E. coli K-12 internal-standard recovery. ▪, transcript abundance; •, gene abundance. The means and SEs for last three time points for panels B and C were used to calculate relationships between rate and ratio. Error bars represent the standard deviation of the mean for four replicates.