Number of MR molecules per cell required to generate the corresponding CH4 production rates per cell (mcrA DNA abundance), calculated using the specific temperature-dependent in vitro activities of M. marburgensis for 4, 15, and 25°C, extrapolated from data reported by Goenrich and colleagues (15) (0.085, 0.28, and 1.6 μmol CH4 min−1 mg−1, respectively). For 30°C, the MR activity of 25°C was used, as CH4 production during the temperature trial was highest at 25 and 30°C.