TABLE 1.
Temperature- and time-dependent total mcrA transcript and gene abundances, total DNA and RNA yields, and mcrA transcript and gene detection limits
| Parameter | Mean (SE) per exptl data set for: |
||
|---|---|---|---|
| Peat stored for 4 mo (n = 15) | Peat stored for 6 mo (n = 21) | Effect-of-temp expt (n = 16) | |
| mcrA transcript abundance/ng nucleic acid | 6.6 × 103 (1.7 × 103) | 3.9 × 102 (8.5 × 101) | 6.1 × 102 (9.7 × 101) |
| mcrA transcript abundance/g soil | 6.7 × 107 (1.2 × 107) | 1.1 × 107 (3.9 × 106) | 8.5 × 106 (1.4 × 106) |
| mcrA gene abundance/ng nucleic acid | 4.5 × 103 (4.3 × 102) | 2.4 × 103 (2.9 × 102) | 3.3 × 102 (4.6 × 101) |
| mcrA gene abundance/g soil | 1.7 × 108 (4.2 × 107) | 3.6 × 107 (3.9 × 106) | 3.4 × 107 (3.9 × 106) |
| Internal-standard-corrected mcrA transcript abundance/g soil | 4.8 × 108 (8.1 × 107) | ||
| Internal-standard-corrected mcrA gene abundance/g soil | 9.1 × 108 (1.5 × 108) | ||
| Detection limit for mcrA transcript abundance/g soil | 1.9 × 103 | 6.5 × 103 | 3.1 × 103 |
| Detection limit for mcrA gene abundance/g soil | 3.2 × 104 | 1.5 × 104 | 1.5 × 105 |
| Total DNA yield (μg/g) | 32 (2.0) | 15 (0.7) | 152 (12.0) |
| Total RNA yield (μg/g) | 26 (0.8) | 14 (0.8) | 17 (1.0) |