TABLE 1.
Molecular genotyping of C. muris (Cm) and C. parvum (Cp) in a mixture of live and heat-killed oocysts using CryptoPMA-PCRa
| Oocyst |
Ratio | Oocyst detected in exptb: |
Livec | Deadc | |||
|---|---|---|---|---|---|---|---|
| Live | Heat-killed | 1 | 2 | 3 | |||
| Cp | Cm | 0:100 | − | − | − | 0/3 | 0/3 |
| Cp | Cm | 1:99 | − | − | − | 0/3 | 0/3 |
| Cp | Cm | 10:90 | Cp | − | Cp | 2/3 | 0/3 |
| Cp | Cm | 50:50 | Cp | Cp | Cp | 3/3 | 0/3 |
| Cp | Cm | 100:0 | Cp | Cp | Cp | 3/3 | 0/3 |
| Cm | Cp | 0:100 | − | − | − | 0/3 | 0/3 |
| Cm | Cp | 1:99 | Cm | − | − | 1/3 | 0/3 |
| Cm | Cp | 10:90 | Cm | Cm | Cm | 3/3 | 0/3 |
| Cm | Cp | 50:50 | Cm | Cm | Cm | 3/3 | 0/3 |
| Cm | Cp | 100:0 | Cm | Cm | Cm | 3/3 | 0/3 |
a
The SSU rRNA gene and the hsp70 gene were amplified and sequenced.
b
−, no PCR products detected.
c
Number of experiments in which PCR products from live or dead oocysts were detected/total number of experiments.