FIG. 2.
(A) 3-D solution space of mutant TCS099/pLOI297 with the ethanol yield expressed as a function of the biomass yield and oxygen consumption on glycerol. This solution space of the mutant is enclosed in the area constructed from the lines connecting clusters of EMs and situated on the shaded plane. (B) Projection of the 3-D solution space of the mutant on the 2-D space of the ethanol and biomass yields. The inverse relationship of the ethanol and biomass yields on glycerol of the mutant is indicated by a perfect-fit line connecting clusters of EMs as follows: YEtOH/GLY = −0.64·YX/GLY + 0.50 (R2 = 1), where YEtOH/GLY is the ethanol yield on glycerol (in g ethanol/g glycerol) and YX/GLY is the biomass yield on glycerol (in g biomass/g glycerol). (C) Projection of the 3-D solution space of the mutant on the 2-D space of the ethanol yield and the oxygen consumption on glycerol. In each panel, the circles, filled triangles, and squares indicate clusters of EMs, while the open triangles and open diamonds indicate experimental data for mutant TCS099/pLOI297 and TCS099/pLOI297 evolved mutants, respectively, for different kLa values. The open diamond located at the point with the lowest ethanol yield and highest biomass yield indicates the result of the experiment conducted with an elevated kLa (2/min).