TABLE 2.
Predicted ethanol yields for mutant TCS099/pLOI297 and its evolved derivatives
| Strain | kLa (/min) | Biomass yield (g/g)a | Exptl ethanol yield (g/g) | Predicted ethanol yield (g/g)b |
|---|---|---|---|---|
| TCS099/pLOI297 | 0.15 | 0.09 ± 0.00 | 0.37 ± 0.01 | 0.44 ± 0.00 |
| TCS099/pLOI297 | 0.2 | 0.12 ± 0.03 | 0.31 ± 0.01 | 0.42 ± 0.02 |
| TCS099/pLOI297 | 0.3 | 0.11 ± 0.01 | 0.34 ± 0.00 | 0.43 ± 0.00 |
| TCS099 e50rep1/pLOI297 | 0.15 | 0.07 ± 0.00 | 0.49 ± 0.03 | 0.45 ± 0.00 |
| TCS099 e50rep1/pLOI297 | 0.2 | 0.10 ± 0.01 | 0.46 ± 0.01 | 0.44 ± 0.01 |
| TCS099 e50rep1/pLOI297 | 0.3 | 0.07 ± 0.01 | 0.45 ± 0.01 | 0.45 ± 0.00 |
a
Experimentally determined.
b
The predicted ethanol yields were determined by using the equation YEtOH/GLY = −0.64·YX/GLY + 0.50, which was derived from the overall stoichiometric equations for the efficient ethanol production pathways (Fig. 2B).