TABLE 2.
Primers used in this study
| Primer | Descriptiona | Sequence (5′-3′) |
|---|---|---|
| 1293 | 50-bp overhang mrk knockout forward primer | TCTTCTCTCTGCAGCAATGGCAACCGCGTTTTTTGGCATGACTGCTGCCCGTGTAGGCTGGAGCTGCTTCG |
| 1294 | 50-bp overhang mrk knockout reverse primer | GGTGTGAGCGGGATAGTTGTCTGAGTCACAGGCAGTTTCCTCTTCACCAGCATATGAATATCCTCCTTAG |
| 1295 | Screening forward primer (mrk) | GGCAGCATAACCGAACAAAT |
| 1296 | Screening reverse primer (mrk) | TAAATTTTCTGCGGCAAACC |
| 1297 | 50-bp overhang pilX knockout forward primer | GTGGAATATTTATGTTATCTACTTCTACTTTTCTTGCGCTTGCCATGCAATGTGTAGGCTGGAGCTGCTTCG |
| 1298 | 50-bp overhang pilX knockout reverse primer | TTTAATACTCATGATAATATCAATATTACTCAGTACACGTCTTAAAATGACATATGAATATCCTCCTTAG |
| 1299 | Screening forward primer (pilX) | CCGCAACGCAATGTACTCTA |
| 1300 | Screening reverse primer (pilX) | CGTTTTTCCGTTCAGGAAGT |
| 1301 | 50-bp overhang Cmr tag forward primer | AACGGGAAAACGAAAACAACAGATGAAAAGACACATACATTCAATACAGCGTGTAGGCTGGAGCTGCTTCG |
| 1302 | 50-bp overhang Cmr tag reverse primer | TTGCACTGGAGTTGATAATGGCCTCAATGCACAAATTGAGGTAAATTTATGTGTAGGCTGGAGCTGCTTCG |
| 1303 | Screening forward primer (tagging) | AGCAGTCCTGCAACCTAAGC |
| 1304 | Screening reverse primer (tagging) | TAATGGGCGTCCTAAACTGG |
| 1305 | GFP-kan mrk knockout forward primer | TCTTCTCTCTGCAGCAATGGCAACCGCGTTTTTTGGCATGACTGCTGCCCTAGGGCGAATTGACATTGTG |
| 1306 | GFP-kan mrk knockout reverse primer | GGTGTGAGCGGGATAGTTGTCTGAGTCACAGGCAGTTTCCTCTTCACCAGTGGACCAGTTGGTGATTTTG |
| 1307 | GFP-kan pilX knockout forward primer | GTGGAATATTTATGTTATCTACTTCTACTTTTCTTGCGCTTGCCATGCAATTAGGGCGAATTGACATTGTG |
| 1308 | GFP-kan pilX knockout reverse primer | TTTAATACTCATGATAATATCAATATTACTCAGTACACGTCTTAAAATGATGGACCAGTTGGTGATTTTG |
| 1309 | GFP-kan tagging forward primer | AACGGGAAAACGAAAACAACAGATGAAAAGACACATACATTCAATACAGCTAGGGCGAATTGACATTGTG |
| 1310 | GFP-kan tagging reverse primer | TTGCACTGGAGTTGATAATGGCCTCAATGCACAAATTGAGGTAAATTTATTGGACCAGTTGGTGATTTTG |
| pCO_F | pCO13 internal screening forward primer (kan region) | CGAAAGATCCCAACGAAAAG |
| pCO13-R2 | pCO13 internal screening reverse primer (GFP region) | TCGATAGATTGTCGCACCTG |
| pCO13-F3 | pCO13 external screening forward primer (GFP region) | CAGCAACACCTTCTTCACGA |
| pCO13-R3 | pCO13 external screening reverse primer (kan region) | TCACCTTCACCCTCTCCACT |
a
GFP, green fluorescent protein.