FIG. 3.

Biological control phenotypes of wild-type Rahnella aquatilis HX2 and its Tn5 mutants MH15 and MH16, deletion mutant HX2Δgdh, and complemented mutants CMH15(p465), CMH15(pqq), CMH16(p104), CMH16(gdh), and CHX2Δgdh on grapevine (Vitis vinifera cv. Muscat Hamburg) shoots. Shoots were inoculated by placing 10-μl drops of bacterial strains (10⁸ CFU ml⁻¹), alone or in equal-number mixtures, at sites in which wounds of 1.0-cm longitudinal incisions were made with a sterile scalpel. Tumors were readily apparent 7 weeks after inoculation. The treatments were SDW alone (a), Agrobacterium vitis K308 alone (b), HX2 plus K308 (c), MH16 plus K308 (d), CMH16(p104) plus K308 (e), CMH16(gdh) plus K308 (f), HX2Δgdh plus K308 (g), CHX2Δgdh plus K308 (h), MH15 plus K308 (i), CMH15(p465) plus K308 (j),CMH15(pqq) plus K308 (k), and MH15 plus PQQ (l).