TABLE 2.
Primers used in this study
| Primer | DNA sequencea |
|---|---|
| Primers for cloning Tn5-flanking sequences | |
| PI | 5′-AGATCTGATCAAGAGACAG-3′ |
| PO | 5′-ACTTGTGTATAAGAGTCAG-3′ |
| Primers for amplifying sequences to screen cosmid clones | |
| P1 | 5′-TGTCCGTATTGCTCGAACCC-3′ |
| P2 | 5′-CAGCACGAAGTTCAGCACC-3′ |
| P3 | 5′-CACGTCGTATCATTCTGCC-3′ |
| P4 | 5′-CTTCATAACGCAGTTT GTGG-3′ |
| Primers for amplifying sequences used in in-frame deletion mutagenesis | |
| P16L1(EcoRI) | 5′-ATGAATTCGTGGAACCTGACATCGC-3′ |
| P16L2(BamHI) | 5′-ATGGATCCAGATACCGCCGACAAG-3′ |
| P16R1(BamHI) | 5′-ATGGATCCTACCTGCGTGCATTTGA-3′ |
| P16R2(PstI) | 5′-TAACTGCAGTCTGACCCTGGAACCTC-3′ |
| Primers for amplification of gdh | |
| Gdh-up (PstI) | 5′-AATCTGCAGCATGGGCTACGCTTCTCTTGA-3′ |
| Gdh-down (EcoRI) | 5′-ATGAATTCGATTACGCCCGCATCTTTG-3′ |
a
Underlined bases are restriction enzyme cut sites.