TABLE 1.
Strains and plasmids utilized in this study
| Strain/plasmid | Description/relevant genotypea | Reference/source |
|---|---|---|
| V. cholerae strains | ||
| O395 | Classical, Ogawa, Smr | 43 |
| C6706 Str2 | El Tor, Inaba, Smr | 44 |
| N16961 | El Tor, Inaba, Smr | 37 |
| KSK1789 | C6706 ΔhapR, Smr | 29 |
| BAJ2 | O395 ΔlacZ ΔgbpA::lacZ, Smr | 26 |
| BAJ21 | C6706 ΔlacZ ΔgbpA::lacZ, Smr | This study |
| BAJ118 | C6706 ΔlacZ ΔgbpA::lacZ ΔhapR, Smr | This study |
| BAJ332 | C6706 ΔprtV, Smr | This study |
| BAJ334 | C6706 ΔhapA, Smr | This study |
| BAJ338 | C6706 ΔhapA ΔprtV, Smr | This study |
| BAJ380 | C6706 ΔlacZ ΔgbpA::lacZ ΔprtV, Smr | This study |
| BAJ382 | C6706 ΔlacZ ΔgbpA::lacZ ΔhapA, Smr | This study |
| BAJ383 | C6706 ΔlacZ ΔgbpA::lacZ ΔhapA ΔprtV, Smr | This study |
| GK999 | N16961 HapRC6706, Smr | This study |
| E. coli strains | ||
| S17-1λpir | thi pro recA hsdR [RP4-2 Tc:Mu-Km::Tn7] λpir Tpr Smr | 13 |
| TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Smr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pBAD-TOPO | Expression vector, PBAD promoter | Invitrogen |
| pKAS154 | Allelic exchange vector | 28 |
| pBro65 | pKAS154 ΔhapA, Kmr | This study |
| pBro63 | pKAS154 ΔprtV, Kmr | This study |
| pBro60 | pBAD-TOPO hapA, Apr | This study |
| pBro62 | pBAD-TOPO prtV, Apr | This study |
| pKAS187 | ΔhapR | 29 |
| pGKK288 | HapRC6706 | This study |
a
Kmr, kanamycin resistance; Smr, streptomycin resistance; Apr, ampicillin resistance; Tpr, trimethoprim resistance.