TABLE 2.
Genotypic and phenotypic characteristics of Fos-R mutants and their positions in the glpT gene in 10 independent Fos-R mutantsa
| Strain | Fos MIC (μg/ml) for strain harboring plasmid: |
Resistance mutation | |
|---|---|---|---|
| pBBR1MCS-3 | pBBR-glpT | ||
| PA14 | 8 | 8 | |
| glpT::MAR2xT7 | 1,024 | 8 | Insertional inactivation |
| FosR-7 | 1,024 | 8 | A59 deletion, 1 bp (frameshift); stop TGA318 |
| FosR-9 | 1,024 | 8 | C219ATCGC225 deletion, 6 bp; Δ (Ile74-Ala75); TM2 |
| FosR-1 | 1,024 | 8 | A220TCGCC226 deletion, 6 bp; Δ (Ile74-Ala75); TM2 |
| FosR-2 | 1,024 | 8 | T365CATGTT371 deletion, 7 bp (frameshift); stop TGA507 |
| FosR-3 | 1,024 | 8 | G to A; Gly137(GG410C)→ Asp (GAC); TM4 |
| FosR-5 | 1,024 | 8 | G596 deletion, 1 bp (frameshift); stop TAG600 |
| FosR-10 | 512 | 8 | C975GG insertion, 3 bp; +Gly326; TM9 |
| FosR-4 | 1,024 | 8 | A to C; Thr336 (A1006CC)→ Pro (CCC); TM9 |
| FosR-8 | 1,024 | 8 | C to G; Tyr362 (TAC1086)→ stop TAG; TM10 |
| FosR-6 | 512 | 8 | G to A; Met 366(ATG1098)→ Ile (ATA); TM10 |
a
The levels of resistance when harboring the empty vector pBBR1MCS-3 or the complementing plasmid pBBR-glpT also are shown. The mutants are ordered in the table according to the position of their mutations in the glpT gene.