TABLE 1.
81-176 mutants used in this study
| Gene(s) | NCTC 11168 no. | CJJ81176 no. | Function | Source or reference |
|---|---|---|---|---|
| flgR | 1024 | 1043 | σ54 transcriptional activator | 7 |
| flgS | 0793 | 0814 | Histidine kinase of FlgR | 7 |
| flgE | 0043 | 0025 | Hook protein | 6 |
| fliR | 1179 | 1194 | Export component | 7 |
| fliP | 0820 | 0837 | Export component | This work |
| flaA flaB | 1339/1338 | 1339/1338 | Major and minor flagellins | 4, 7 |
| flhF | 0064 | 0102 | Signal recognition particle; export component | This work |
| flhA | 0882 | 0890 | Export component | 7 |
| flhB | 0335 | 0357 | Export component; substrate specificity switch | 6 |
| pseA | 1316 | 1333 | Synthesis of PseAm | 39 |
| pseB | 1293 | 1310 | Dehydratase that converts UDP-GlcNAc to UDP-2-acetamidino-2,6-dideoxy-β-l-arabino-hexos-4-ulose; first enzyme in PseAc synthesis | 9 |
| pseC | 1294 | 1311 | Converts the product of PseB to UDP-4-amino-4,6-dideoxy-β-l-AltNAc; second enzyme in PseAc synthesis | 9 |
| pseD | 1333 | 1336 | PseAm transferase | 9 |
| pseE | 1337 | 1337 | PseAc transferase | 9 |