TABLE 1.

Oligodeoxynucleotides used to change specific codons in the tnaC genes examined in this study

tnaC source	Change	Oligodeoxynucleotide sequence
Proteus vulgaris	K33R	5′-CGCCTTCTTCTTTCCTCGTAAATAATCCCTTTTCTATAC-3′
		5′-GTATAGAAAAGGGATTATTTACGAGGAAAGAAGAAGGCG-3′
	K33I	5′-CGCCTTCTTCTTTCCTATAAAATAATCCCTTTTC-3
		5′-GAAAAGGGATTATTTTATAGGAAAGAAGAAGGCG-3′
	K33R-K34R	5′-CGCCTTCTTCTTTCCTCGTCGTTAATCCCTTTTCTATAC-3′
		5′-GTATAGAAAAGGGATTAACGACGAGGAAAGAAGAAGGCG-3′
	K33I-K34I	5′-CGCCTTCTTCTTTCCTATAATATAATCCCTTTTCTATAC-3′
		5′-GTATAGAAAAGGGATTATATTATAGGAAAGAAGAAGGCG-3′
	ΔK33-34	5′-CGCCTTCTTCTTTCCTTAATCCCTTTTCTATAC-3′
		5′-GTATAGAAAAGGGATTAAGGAAAGAAGAAGGCG-3′
	TAA35TGA	5′-CTTCTTCTTTCCTAAAAAATGATCCCTTTTCTATACATATC-3′
		5′-GATATGTATAGAAAAGGGATCATTTTTTAGGAAAGAAGAAG-3′
	W20R	5′-CGTCAGATTGAAGAAACGGTTTAATATTGA-3′
		5′-GAGTCAATATTAAACCGTTTCTTCAATCTGACG-3′
	D24A	5′-GAAATGGTTTAATATTGCCTCTGAACTCGCCTTC-3′
		5′-GAAGGCGAGTTCAGAGGCAATATTAAACCATTTC-3′
	L27A	5′-ATATTGACTCTGAAGCCGCCTTCTTCTTTCC-3′
		5′-GGAAAGAAGAAGGCGGCTTCAGAGTCAATAT-3′
	P32A	5′-GAACTCGCCTTCTTCTTTCCTAAAAAATAATCCCTTTTC-3′
		5′-GAAAAGGGATTATTTTTTAGGAAAGAAGAAGGCGAGTTC-3′
Escherichia coli	W12R	5′-GTGTGACCTCAAAATGGTTCAATATTGACAAC-3′
		5′-GTTGTCAATATTGAACCATTTTGAGGTCACAC-3′
	D16A	5′-CTCAAAATGGTTCAATATTGCCAACAAAATTGTCGATCACC-3′
		5′-GGTGATCGACAATTTTGTTGGCAATATTGAACCATTTTGAG-3′
	P24A	5′-GTCGATCACCGCGCTTGATTTGCCTTC-3′
		5′-GAAGGCAAATCAAGCGCGGTGATCGAC-3′