FIG. 2.

Comparison of corneocyte binding of WT, Fib⁺, and Fib⁻ cells and contribution of the Aap A domain to adhesion of NCTC 11047 to corneocytes. (a) Mean number of NCTC 11047 WT, Fib⁺, and Fib⁻ cells attached to corneocytes after 2 h of incubation. (b) Blocking of adhesion of NCTC 11047 WT (filled symbols) and Fib⁺ (hollow symbols) cells to corneocytes using specific recombinant Aap domains. Corneocytes were preincubated with increasing concentrations of rAap_A-Dom (circles), 1 μM rAap_B-rep (squares), or 1 μM rAap_A-reps (triangles), and the adhesion of NCTC 11047 WT and Fib⁺ cells is shown as a percentage of original binding. Results represent the means and standard errors for at least two experiments.