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. 2009 Sep 11;191(22):7007–7016. doi: 10.1128/JB.00764-09

FIG. 3.

FIG. 3.

Surface expression of fibrillar Aap on L. lactis MG1363 pUB1000aap6high cells. (a) Western blot of cell wall proteins probed with anti-Aap A-domain antiserum. (b and c) TEM of L. lactis MG1363 strains negatively stained with 2% methylamine tungstate. (d and e) TEM of L. lactis MG1363 strains negatively stained and immunogold labeled with anti-Aap A-domain antiserum and 10-nm gold-conjugated secondary antibody. (b) MG1363 pUB1000aap6high cell showing fibrils projecting from the cell wall. (c) MG1363 pUB1000 cell showing a smooth cell surface. (d) MG1363 pUB1000aap6high cell showing gold labeling evenly distributed over the cell. (e) MG1363 pUB1000 cell showing only a small amount of nonspecific gold labeling.