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. 2009 Sep 11;191(22):7007–7016. doi: 10.1128/JB.00764-09

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Copyright © 2009, American Society for Microbiology

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FIG. 4. — Role of the A domain of Aap in adhesion to corneocytes. (a) Western blot of cell wall proteins from MG1363 containing pUB1000, pUB1000aap6high, and pUB1000aap6highT and probed with anti-Aap B-repeat antiserum. (b) Mean numbers of L. lactis MG1363 pUB1000aap6high, pUB1000aap6highT, and pUB1000 control cells that adhered to corneocytes. (c) Corneocytes were preincubated with increasing concentrations of rAap_A-Dom (circles), 1 μM rAap_A-reps (triangles), or 1 μM rAap_B-rep (squares), and the mean numbers of MG1363 pUB1000aap6high cells that adhered to corneocytes are shown. The results represent the means and standard errors for three experiments.