FIG. 6.

Influence of numbers of B repeats in Aap on adhesion to corneocytes. (a) Agarose gel showing sizes of aap in pUB1000aap2, pUB1000aap4, pUB1000aap5, pUB1000aap6, and pUB1000aap7. The expected sizes of PCR products for pUB1000aap with 2, 4, 5, 6, and 7 B repeats are 1,652, 2,420, 2,804, 3,188 and 3,572 bp, respectively. PCR was performed using primers Aap53-608rc and aapR (Table 1). (b) Whole-cell dot immunoblot of MG1363 pUB1000 expressing different lengths of Aap showing surface expression levels of Aap. Serial dilutions of cells were applied onto a PVDF membrane and probed with anti-Aap A-domain antiserum. (c) Mean number of MG1363 pUB1000aap cells expressing different lengths of Aap that adhered to corneocytes.