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nanS is required for E. coli to grow on Neu5,9Ac2 as the sole carbon source. Overnight cultures of BW30270 and its isogenic nanS mutant derivative grown on glycerol as the carbon source were diluted 1:50 into fresh medium containing either glycerol or Neu5,9Ac2 as follows: BW30270 with glycerol (closed squares); nanS mutant with glycerol (closed triangles); BW30270 with Neu5,9Ac2 (open squares); nanS mutant with Neu5,9Ac2 (open triangles).
