FIG. 3.

Growth of the wild type and the yjhS and nanA mutants on different sialic acids. In panels A to D, the indicated wild-type strains (open boxes) and their isogenic yjhS deletion derivatives (shaded boxes) were diluted 50-fold from stationary-phase cells grown in M63 medium plus glycerol (0.4%) into 0.1 ml of M63 medium containing 0.1% of the indicated sialic acids or glycerol in 96-well plates. (A) Growth on Neu5Ac. (B) Growth on Neu5Gc. (C) Growth on Neu5,9Ac₂. (D) Growth on glycerol. The plate was incubated without shaking at 37°C for 16 h, and A₆₀₀ values were recorded with a microplate reader. Data represent the means of three independent experiments ± standard deviations. (E) S. enterica serovar Typhimurium wild-type strain 14028, nanA strain LT2 derivative M2, and E. coli nanA mutant EV78 were diluted 60-fold into 0.5 ml of M63 medium containing 0.4% glycerol, 0.1% Neu5Ac, or 0.1% Neu5,9Ac₂. Cultures were grown at 37°C with vigorous aeration; the A₆₀₀ values were recorded after 7 h.