FIG. 4.

Utilization of Neu5Ac or Neu5,9Ac₂ by BW30270 and its yjhS deletion derivative growing simultaneously in the presence of glycerol. BW30270 or its yjhS derivative was diluted 60-fold into 0.5 ml of M63 medium plus glycerol containing either 0.1% Neu5Ac or Neu5,9Ac₂ and incubated at 37°C with vigorous aeration for 7 h unless indicated otherwise. (A) Neu5,9Ac₂ incubated without cells. (B) Neu5Ac incubated without cells. (C) Wild type grown with Neu5Ac. (D) Mutant grown with Neu5Ac. Note that the minor peak eluting at about 10 min as shown in these panels and in the succeeding panel is likely to represent a trace amount of 2-deoxy-d-manno-octulosonic acid derived from sloughed lipopolysaccharide during the incubation that was not removed by the centrifugation step used to produce the spent culture media for the analyses (22). (E) Wild type grown with Neu5,9Ac₂. (F) Mutant grown with Neu5,9Ac₂. (G) Neu5,9Ac₂ incubated without cells for 17 h. (H) Mutant grown with Neu5,9Ac₂ for 17 h.