Skip to main content
. 2009 Sep 11;191(22):6865–6876. doi: 10.1128/JB.00818-09

FIG. 8.

FIG. 8.

DNase I footprinting analysis of wild-type CodY binding to the ilvB promoter in the presence of BCAAs and BCAA-related compounds. All reaction mixtures contained 160 nM wild-type CodY. (A) Reaction mixtures shown in lanes 2 to 4, lanes 5 to 7, and lanes 8 to 10 contained increasing concentrations (Conc) of l-valine, 3-methyl-2-oxobutyric acid, and 4-methyl-2-oxovaleric acid, respectively, as indicated above the gel. The samples in the leftmost and rightmost lanes did not contain BCAAs or BCAA-related compounds. (B) The samples in lanes 2 and 3, lanes 4 and 5, and lanes 6 and 7 contained increasing concentrations of l-valine, 3-methyl butyric acid, and 4-methyl valeric acid, respectively, as indicated above the gel. The sample in the leftmost lane did not contain BCAAs or BCAA-related compounds. (C) The samples in lanes 2 to 4, lanes 5 to 7, and lanes 8 to 10 contained increasing concentrations of l-valine, d-valine, and l-2-amino butyric acid, respectively, as indicated above the gel. The sample in lane 1 did not contain BCAAs or BCAA-related compounds.