TABLE 1.
Strains and plasmids
| Strain and plasmid | Genotype | Reference or source |
|---|---|---|
| S. meliloti | ||
| Rm1021 | SU47 Smr | 42 |
| MGM044 | Rm1021/pTE3 | This study |
| MGM312 | Rm1021/pMW23 | This study |
| MGM522 | Rm1021/pTE3, pMS04 | This study |
| MGM523 | Rm1021/pMW23, pMS04 | This study |
| MGM490 | Rm1021/pTE3, pMB393 | This study |
| MGM491 | Rm1021/pMW23, pMB393 | This study |
| MGM024 | rkpA::Nm | This study |
| MGM050 | rkpA::Nm/pTE3 | This study |
| MGM314 | rkpA::Nm/pMW23 | This study |
| MGM003 | rkpG::pDW33 | This study |
| MGM048 | rkpG::pDW33/pTE3 | This study |
| MGM388 | rkpG::pDW33/pMW23 | This study |
| MGM445 | rkpH::pDW33 | This study |
| MGM454 | rkpH::pDW33/pTE3 | This study |
| MGM455 | rkpH::pDW33/pMW23 | This study |
| MGM418 | rkpI::pDW33 | This study |
| MGM423 | rkpI::pDW33/pTE3 | This study |
| MGM424 | rkpI::pDW33/pMW23 | This study |
| MGM419 | rkpJ::pDW33 | This study |
| MGM425 | rkpJ::pDW33/pTE3 | This study |
| MGM426 | rkpJ::pDW33/pMW23 | This study |
| MGM532 | rkpA::Nm/pTE3, pMS04 | This study |
| MGM508 | rkpA::Nm/pTE3, pMS04::rkpGH | This study |
| MGM533 | rkpA::Nm/pMW23, pMS04 | This study |
| MGM509 | rkpA::Nm/pMW23, pMS04::rkpGH | This study |
| MGM528 | rkpG::pDW33/pTE3, pMS04 | This study |
| MGM510 | rkpG::pDW33/pTE3, pMS04::rkpGH | This study |
| MGM529 | rkpG::pDW33/pMW23, pMS04 | This study |
| MGM511 | rkpG::pDW33/pMW23, pMS04::rkpGH | This study |
| MGM530 | rkpH::pDW33/pTE3, pMS04 | This study |
| MGM512 | rkpH::pDW33/pTE3, pMS04::rkpGH | This study |
| MGM531 | rkpH::pDW33/pMW23, pMS04 | This study |
| MGM513 | rkpH::pDW33/pMW23, pMS04::rkpGH | This study |
| MGM395 | rkpG::pDW33 rkpA::Nm | This study |
| MGM400 | rkpG::pDW33 rkpA::Nm/pTE3 | This study |
| MGM402 | rkpG::pDW33 rkpA::Nm/pMW23 | This study |
| MGM464 | rkpH::pDW33 rkpA::Nm | This study |
| MGM465 | rkpH::pDW33 rkpA::Nm/pTE3 | This study |
| MGM466 | rkpH::pDW33 rkpA::Nm/pMW23 | This study |
| MGM427 | rkpI::pDW33 rkpA::Nm | This study |
| MGM429 | rkpI::pDW33 rkpA::Nm/pTE3 | This study |
| MGM430 | rkpI::pDW33 rkpA::Nm/pMW23 | This study |
| MGM428 | rkpJ::pDW33 rkpA::Nm | This study |
| MGM431 | rkpJ::pDW33 rkpA::Nm/pTE3 | This study |
| MGM432 | rkpJ::pDW33 rkpA::Nm/pMW23 | This study |
| MGM403 | kdsB2::pDW33 | This study |
| MGM404 | kdsB2::pDW33/pTE3 | This study |
| MGM405 | kdsB2::pDW33/pMW23 | This study |
| MGM420 | smb20804::pDW33 | This study |
| MGM433 | smb20804::pDW33/pTE3 | This study |
| MGM434 | smb20804::pDW33/pMW23 | This study |
| MGM365 | smb20805::pDW33 | This study |
| MGM383 | smb20805::pDW33/pTE3 | This study |
| MGM385 | smb20805::pDW33/pMW23 | This study |
| MGM494 | kdsB2::pDW33/pMW23, pMB393 | This study |
| MGM486 | kdsB2::pDW33/pMW23, pMB393::smb20805 | This study |
| MGM547 | smb20804::pDW33/pMW23, pMB393 | This study |
| MGM526 | smb20804::pDW33/pMW23, pMB393::smb20805 | This study |
| MGM492 | smb20805::pDW33/pMW23, pMB393 | This study |
| MGM488 | smb20805::pDW33/pMW23, pMB393::smb20805 | This study |
| DKR145 | exoY::pVO155 | 78 |
| MGM081 | exoY::pVO155/pTE3 | This study |
| MGM358 | exoY::pVO155/pMW23 | This study |
| MGM340 | exoY::pVO155 kdsB2::pDW33 | This study |
| MGM352 | exoY::pVO155 kdsB2::pDW33/pTE3 | This study |
| MGM354 | exoY::pVO155 kdsB2::pDW33/pMW23 | This study |
| DKR530 | exoY::pDW33 | This study |
| MGM359 | exoY::pDW33/pTE3 | This study |
| MGM361 | exoY::pDW33/pMW23 | This study |
| MGM349 | exoY::pDW33 rkpA::Nm | This study |
| MGM355 | exoY::pDW33 rkpA::Nm/pTE3 | This study |
| MGM357 | exoY::pDW33 rkpA::Nm/pMW23 | This study |
| Escherichia coli | ||
| DH5α | F− φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1 | 65 |
| MT616 | E. coli pRK600 (conjugation helper strain) | 40 |
| Plasmids | ||
| pCR2.1 | TOPO cloning vector | Invitrogen |
| pVO155 | Insertional inactivation plasmid Nmr | 45 |
| pDW33 | Insertional inactivation plasmid Hygr | 12 |
| pJQ200 | Suicide plasmid GmrsacB | 55 |
| pTE3 | pLAFR containing the trp promoter from Salmonella typhi serovar Typhimurium | 3 |
| pMW23 | pRK404, harbors 4.0-kb PstI fragment containing rkpZ from Rm41 | 79 |
| pMB393 | Broad-host-range cloning vector; Spr | 2 |
| pMS04 | Broad-host-range vector, high-copy-number derivative of pMB393 containing the trp promoter from Salmonella typhi serovar Typhimurium | 35 |
| pMTO001 | pCR2.1::rkpA (2,156-bp internal fragment) | This study |
| pMTO002 | pCR2.1::rkpA (2,156-bp internal fragment, carrying a 1,258-bp deletion) | This study |
| pMG003 | pJQ200::rkpA (2,156-bp internal fragment, carrying a 1,258-bp deletion) | This study |
| pMG006 | pJQ200::rkpA::Nm (2,156-bp internal fragment, a 1,258-bp deletion with insertion of Nmr cassette) | This study |
| pMG043 | pVO155::rkpA (internal fragment) | This study |
| pMG042 | pDW33::rkpG (internal fragment) | This study |
| pMG510 | pDW33::rkpH (internal fragment) | This study |
| pMG511 | pDW33::rkpI (internal fragment) | This study |
| pMG512 | pDW33::rkpJ (internal fragment) | This study |
| pDKR520 | pDW33::kdsB2 (internal fragment) | This study |
| pMG502 | pDW33::smb20804 (internal fragment) | This study |
| pMG503 | pDW33::smb20805 (internal fragment) | This study |
| pDKR530 | pDW33::exoY (internal fragment) | This study |
| pMG060 | pMS04::rkpGH (full length) | This study |
| pMG052 | pMB393::smb20805 (full length) | This study |