TABLE 3.
Flagellar glycosylation locus genes and similarity of predicted proteins to proteins in the microbial genome database
| Strain and gene | Predicted function(s) (E value) | C. difficile homolog(s) |
|---|---|---|
| 630 | ||
| CD0240 (GT1) | β-(1,4)-Glycosyltransferase (6e−29), ExoA homolog (2e−12), GT family 2 (5e−10) | CIP 107932, 23m63, 32g58, 37x79, 63q42, 76w55, 97b34, |
| CD0241 | Phosphoserine-phosphatase-like protein (2e17) | None |
| CD0242 | Phosphocholine cytidylyltransferase (1e−21) | None |
| CD0243 | Methyltransferase (2e−27) | None |
| CD0244 | CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (1e−18), cholinephosphotransferase (3e−7), GT family 2 (3e−6) | None |
| QCD32g58 | ||
| orf1 (GT1) | GT family 2 (2e−57) | 37x79, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf2 (GT2) | GT family 2 (3e−38), methyltransferase (2e−13) | 37x79, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf3 (GT3)a | GT family 2 (9e−73), streptomycin biosynthesis protein (4e−47) | QCD32g58, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf4 | Carbamoyl-phosphate synthase large subunit (6e−38), biotin carboxylase (1e−20) | 37x79, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf5 | Ornithine cyclodeaminase (2e−74) | 37x79, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf6 | FdtA, dTDP-4-keto-6-deoxy-d-glucose-3,4-ketoisomerase (2e−29) | 37x79, 63q42, 97b34, CIP 107932, 23m63,76w55 |
| orf7 | TDP-3-aminoquinovose aldamine (2e−101), glutamine-scyllo-inositol transaminase (4e−133) | 37x79, 63q42, 97b34, CIP 107932, 76w55 |
| orf8 | 3-Amino-5-hydroxybenzoic acid synthase family (5e−119), aminotransferase family (1e−9) | 37x79, 63q42, 97b34, CIP 107932, 76w55 |
a
orf3 protein sequence from C. difficile 37x39 used in Blastp, as potential sequencing errors in QCD32g58 gene cause a truncation in the ORF.