Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Sep 18;191(22):6928–6935. doi: 10.1128/JB.00947-09

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, American Society for Microbiology

PMC Copyright notice

FIG. 3. — (Upper panel) Western immunoblot analysis of MtaC and MtaA proteins expressed from synthetic PmcrB(tetO1)-mtaCBA operons. (Lower panel) Coomassie blue-stained polyacrylamide gel electrophoresis gel run with equal amounts of total protein from each extract. Lane 1, parental strain (WWM 148), from which all mta genes were deleted, grown on TMA; lane 2, PmcrB(tetO1)-mtaC2B2A1 (WWM170) strain, grown on TMA plus tetracycline; lane 3, PmcrB(tetO1)-mtaC2B2A1 (WWM170) strain, grown on methanol plus tetracycline; lane 4, PmcrB(tetO1)-mtaC1B1A1 (WWM176) strain grown on TMA plus tetracycline; lane 5, PmcrB(tetO1)-mtaC1B1A1 (WWM176) strain grown on methanol plus tetracycline; lane 6, PmcrB(tetO1)-mtaC3B3A1 (WWM184) strain, grown on TMA plus tetracycline; lane 7, PmcrB(tetO1)-mtaC3B3A1 (WWM184) strain, grown on methanol plus tetracycline; lane 8, control strain (WWM1), with all mta genes expressed from their native promoters, grown on methanol. For MtaC and MtaA detection, 4 μg and 0.5 μg total protein, respectively, were loaded in each well.