TABLE 3.
Growth characteristicsa of the PmcrB(tetO1)-mtaCBA strains when grown on methanol plus tetracycline
| Relevant genotype | Mean ± SD |
||
|---|---|---|---|
| Generation time (h) | Growth yield (OD600) | Lag time (h) | |
| Wild type | 9.2 ± 0.4 | 0.69 ± 0.04 | 55 ± 1 |
| mta deletion | NGb | NG | NG |
| PmcrB(tetO1)-mtaC1B1A1 | 18.7 ± 4.7 | 0.71 ± 0.08 | 169 ± 25 |
| PmcrB(tetO1)-mtaC1B2A1 | 41.2 ± 11.9 | 0.60 ± 0.05 | 219 ± 6.0 |
| PmcrB(tetO1)-mtaC1B3A1 | 11.5 ± 0.8 | 0.69 ± 0.05 | 261 ± 8 |
| PmcrB(tetO1)-mtaC1B1A2 | 20.1 ± 2.1 | 0.68 ± 0.01 | 169 ± 22 |
| PmcrB(tetO1)-mtaC1B2A2 | 22.2 ± 4.8 | 0.68 ± 0.02 | 169 ± 46 |
| PmcrB(tetO1)-mtaC1B3A2 | 12.5 ± 1.2 | 0.64 ± 0.01 | 264 ± 13 |
| PmcrB(tetO1)-mtaC2B1A1 | 22.1 ± 1.7 | 0.67 ± 0.02 | 126 ± 10 |
| PmcrB(tetO1)-mtaC2B2A1 | 18.4 ± 5.8 | 0.66 ± 0.01 | 230 ± 17 |
| PmcrB(tetO1)-mtaC2B3A1 | 33.1 ± 3.4 | 0.61 ± 0.04 | 222 ± 25 |
| PmcrB(tetO1)-mtaC2B1A2 | 20.4 ± 2.3 | 0.68 ± 0.01 | 129 ± 15 |
| PmcrB(tetO1)-mtaC2B2A2 | 17.8 ± 3.7 | 0.68 ± 0.08 | 267 ± 40 |
| PmcrB(tetO1)-mtaC2B3A2 | 14.7 ± 2.0 | 0.66 ± 0.06 | 262 ± 46 |
| PmcrB(tetO1)-mtaC3B1A1 | 39.4 ± 14.4 | 0.66 ± 0.01 | 192 ± 19 |
| PmcrB(tetO1)-mtaC3B2A1 | 22.2 ± 7.9 | 0.63 ± 0.06 | 239 ± 35 |
| PmcrB(tetO1)-mtaC3B3A1 | 16.0 ± 3.1 | 0.67 ± 0.06 | 233 ± 3 |
| PmcrB(tetO1)-mtaC3B1A2 | 49.0 ± 5.1 | 0.61 ± 0.04 | 189 ± 8 |
| PmcrB(tetO1)-mtaC3B2A2 | 30.3 ± 4.0 | 0.62 ± 0.02 | 251 ± 3 |
| PmcrB(tetO1)-mtaC3B3A2 | 17.1 ± 2.2 | 0.64 ± 0.04 | 256 ± 14 |
a
Growth was measured as described in Materials and Methods by using strains WWM82, WWM148, and WWM168 to WWM185. The growth yield represents the maximum OD600 of the culture. The lag time represents the time needed to reach one-half the maximum OD600. Values represent the means and standard deviations of at least triplicate measurements.
b
NG, no growth.