TABLE 4.
Relative mRNA abundances of the uidA and mta genes when strains were grown on methanol or TMA
| Operon structure | mRNA ratioa |
|||
|---|---|---|---|---|
| uidA | mtaA | mtaC | mtaB | |
| PmcrB(tetO1)-uidA | 1.0 ± 0.2 | NAb | NA | NA |
| PmcrB(tetO1)-mtaC3B3A1 | NA | 9.5 ± 2.1 | 9.5 ± 2.7 | 9.9 ± 3.3 |
| PmcrB(tetO1)-mtaC2B2A1 | NA | 10.3 ± 1.7 | 15.2 ± 6.8 | 13.6 ± 5.8 |
| PmcrB(tetO1)-mtaC1B1A1 | NA | 8.8 ± 2.6 | 7.9 ± 2.1 | 8.9 ± 2.3 |
| PmcrB(tetO1)-mtaC1B1A2 | NA | 9.2 ± 2.2 | NDc | ND |
a
The ratios of mRNA levels from strains grown on methanol to mRNA levels from strains grown on TMA are shown. mRNA abundance was determined by qRT-PCR as described, by using total RNA isolated from strains WWM82 [PmcrB(tetO1)-uidA], WWM184 [PmcrB(tetO1)-mtaC3B3A1], WWM170 [PmcrB(tetO1)-mtaC2B2A1], WWM176 [PmcrB(tetO1)-mtaC1B1A1], and WWM181 [PmcrB(tetO1)-mtaC1B1A2] grown on either HS-methanol or HS-TMA. Tetracycline was added to all cultures to induce expression from the PmcrB(tetO1) promoter. All primers used in this experiment are listed in the supplemental material.
b
NA, not applicable.
c
ND, not determined.