TABLE 1.
Strains used in this study
| Strain | Relevant genotype or descriptiona | Source or constructionb |
|---|---|---|
| 168 | trpC2 | BGSC |
| HB0801 | sigM::kan | 36 |
| HB0803 | sigW::mls | 36 |
| HB0804 | sigX::spc | 36 |
| JH642 | trpC2 pheA1 | 42 |
| NCIB3610 | Prototroph | BGSC |
| NRS1314 | degU::pBL204 (cat) | 53 |
| NRS1434 | trpC2 pheA1 amyE::Pabh-abh::spc | pBL154 → JH642 |
| NRS1893 | trpC2 pheA1 amyE::Phy-spank-abh-lacI (spc) | pNW400 → JH642 |
| NRS1894 | trpC2 pheA1 Δabh::cat | This study |
| NRS1900 | Δabh::cat | SPP1 NRS1894 → NCIB3610 |
| NRS1901 | Δabh::cat amyE::Phy-spank-abh-lacI (spc) | SPP1 NRS1893 → NRS1900 |
| NRS1904 | Δabh::cat amyE::Pabh-abh::spc | SPP1 NRS1434 → NRS1900 |
| NRS1916 | trpC2 pheA1 amyE::Pabh-gfp (cat) | pNW402 → JH642 |
| NRS1917 | amyE::Pabh-gfp (cat) | SPP1 NRS1916 → NCIB3610 |
| NRS1937 | trpC2 pheA1 amyE::Phy-spac-abh-lacI (cat) | pNW403 → JH642 |
| NRS1964 | trpC2 pheA1 amyE::Phy-spankslrR-lacI (spc) | pNW407 → JH642 |
| NRS1965 | amyE::Phy-spank-slrR-lacI (spc) | SPP1 NRS1964 → NCIB3610 |
| NRS1966 | Δabh::cat amyE::Phy-spankslrR-lacI (spc) | SPP1 NRS1964 → 1900 |
| NRS1968 | trpC2 sacA::PslrR-gfp-lacI (kan) | pNW408 → 168 |
| NRS2241 | trpC2 pheA1sacA::PepsA-gfp (kan) | pNW502 → JH642 |
| NRS2242 | sacA::PepsA-gfp (kan) | SPP1 NRS2241 → NCIB3610 |
| NRS2388 | trpC2 sacA::PyqxM-gfp (kan) | pNW602 → 168 |
| NRS2394 | sacA::PyqxM-gfp (kan) | SPP1 NRS2388 → NCIB3610 |
| NRS2547 | Δabh::cat sacA::PepsA-gfp (kan) | SPP1 NRS1894 → NRS2242 |
| NRS2549 | Δabh::cat sacA::PyqxM-gfp (kan) | SPP1 NRS1894 → NRS2394 |
| NRS2550 | sacA::PslrR-gfp-lacI (kan) | SPP1 NRS1968 → NCIB3610 |
| NRS2551 | Δabh::cat sacA::PslrR-gfp-lacI (kan) | SPP1 NRS1968 → NRS1900 |
| NRS2552 | Δabh::cat amyE::Phy-spank-abh-lacI (spc) sacA::PslrR-gfp-lacI (kan) | SPP1 NRS1968 → NRS1901 |
| NRS2553 | Δabh::cat amyE::Pabh-abh::spc sacA::PslrR-gfp-lacI (kan) | SPP1 NRS1968 → NRS1904 |
| NRS2556 | Δabh::cat amyE::Pabh-abh::spc sacA::PepsA-gfp (kan) | SPP1 NRS1434 → NRS2547 |
| NRS2557 | Δabh::cat amyE::Pabh-abh::spc sacA::PyqxM-gfp (kan) | SPP1 NRS1434 → NRS2549 |
| NRS2562 | amyE::Phy-spank-slrR-lacI sacA::PyqxM-gfp (kan) | SPP1 NRS1964 → NRS2394 |
| NRS2566 | amyE::Phy-spank-slrR-lacI sacA::PslrR-gfp (kan) | SPP1 NRS1964 → NRS2550 |
| NRS2571 | sigW::mls | SPP1 HB0803 → NCIB3610 |
| NRS2572 | sigX::spc | SPP1 HB0804 → NCIB3610 |
| NRS2574 | sigW::mls amyE::Phy-spac-abh-lacI (cat) | SPP1 NRS1937 → NRS2571 |
| NRS2575 | sigX::spc amyE::Phy-spac-abh-lacI (cat) | SPP1 NRS1937 → NRS2572 |
| NRS2576 | sigM::kan amyE::Pabh-gfp (cat) | SPP1 NRS1916 → NRS2570 |
| NRS2577 | sigX::spc amyE::Pabh-gfp (cat) | SPP1 NRS1916 → NRS2572 |
| NRS2578 | sigW::mls amyE::Pabh-gfp (cat) | SPP1 NRS1916 → NRS2571 |
| NRS2605 | Δabh::cat amyE::Phy-spank-slrR-lacI (spc)sacA::PepsA-gfp (kan) | SPP1 NRS1964 → NRS2547 |
| NRS2606 | Δabh::cat amyE::Phy-spank-slrR-lacI (spc)sacA::PyqxM-gfp(kan) | SPP1 NRS1964 → NRS2549 |
| NRS2716 | degU::pBL204 (cat) sacA::PslrR-gfp-lacI (kan) | SPP1 NRS1314 → NRS2550 |
a
Drug resistance cassettes are indicated as follows: cat, chloramphenicol resistance; kan, kanamycin resistance; mls, lincomycin-erythromycin resistance; spc, spectinomycin resistance.
b
The direction of strain construction is indicated with DNA or phage (SPP1) (→) recipient strain. BGSC is the Bacillus Genetic Stock Center.