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Degradation of TfR and integrin-related molecules (paxillin and FAK) by MVs. HeLa and IHGE cells were incubated with wild-type and gingipain-null MVs at 37°C for 15 min in serum-free medium. After a wash, the cells were incubated in serum-containing medium for the indicated time periods after addition of MVs. The degradation of TfR, paxillin, and FAK was analyzed at each time point by an immunoblotting method.
