FIG. 5.

Fluorescence microscopy analysis of TfR and paxillin in cells incubated with MVs. (A) HeLa cells were incubated with wild-type and gingipain-null MVs (green) at 37°C for 15 min in serum-free medium. After a wash, the cells were incubated for 45 min in serum-containing medium. Distribution of TfR (white) and paxillin (white) was analyzed by confocal microscopy. (B) Following incubation with MVs for 15 min in serum-free medium, the cells were washed and incubated with Alexa Fluor 594-conjugated transferrin (white) at 37°C for 45 min in serum-containing medium. For fluorescence microscopy, cells were processed for staining with DAPI (4′,6-diamidino-2-pheylindole; blue). At least 20 cells were analyzed in each experiment, with three independent experiments performed. Bars (A and B) = 20 μm.