FIG. 6.

Coexistence of Rgp and fimbriae in the cellular plasma membrane fraction. HeLa cells were incubated with or without wild-type MVs at 37°C for 15 min in serum-free medium. After a wash, the cells were incubated at 37°C for 45 min in serum-containing medium. Separation of basic subcellular fractions was performed, and each was labeled separately with antibodies against fimbriae, Rgp, TfR (marker of insoluble fraction), and aldolase (marker of soluble fraction). The cellular homogenate was separated into supernatants (S100) and pellets (P100), as described in Materials and Methods.