TABLE 2.
Primer | Sequence (5′-3′)a | Description |
---|---|---|
MM727 | CGTTGGAGGATTGCATGCAAAAACTTTCCG | Forward primer for construction of pTW429 (first ilvI fragment) |
MM512 | GAGAATGCATCTCCACCAATGTATAAAACCG | Reverse primer for construction of pTW429 (first ilvI fragment) |
MM513 | AACCGTTATGCATTATATTCCGATTGTGGG | Forward primer for construction of pTW429 (second ilvI fragment) |
MM734 | CTGATGTCGACCTACGCATCTGTTCTC | Reverse primer for construction of pTW429 (second ilvI fragment) |
MM668 | GTTGTGTGGAATTCTGAGCGGATAAC | Forward primer for construction of pRL100 (Kan promoter) |
MM710 | CCCATTGGTACCCATATAAATCAGCATC | Reverse primer for construction of pRL100 (Kan promoter) |
MM744 | CAATAAACCCGTCGACCAATCAAATCC | Forward primer for construction of pRL103 (full ilvIH operon plus promoter) |
MM745 | CGTCATCTAGAGTTATAAAGCAATTAGGGT | Reverse primer for construction of pRL103 (full ilvIH operon plus promoter) |
MM588 | CCATGCCGCGTGAATGA | 16S forward Q-PCR primer |
MM589 | TTCCTCGCTACCGAAAGAACTT | 16S reverse Q-PCR primer |
MM590 | TGTCGGTCAGCACCAAATGT | ilvI forward Q-PCR primer |
MM591 | GCGACGAGGTTTTTCAAACG | ilvI reverse Q-PCR primer |
MM592 | AATTGCTTGAAGCACCGCTATT | lrp forward Q-PCR primer |
MM593 | CGTCCGGCTTACCTCTGACT | lrp reverse Q-PCR primer |
Restriction sites inserted by PCR are underlined.