TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | Description |
|---|---|---|
| MM727 | CGTTGGAGGATTGCATGCAAAAACTTTCCG | Forward primer for construction of pTW429 (first ilvI fragment) |
| MM512 | GAGAATGCATCTCCACCAATGTATAAAACCG | Reverse primer for construction of pTW429 (first ilvI fragment) |
| MM513 | AACCGTTATGCATTATATTCCGATTGTGGG | Forward primer for construction of pTW429 (second ilvI fragment) |
| MM734 | CTGATGTCGACCTACGCATCTGTTCTC | Reverse primer for construction of pTW429 (second ilvI fragment) |
| MM668 | GTTGTGTGGAATTCTGAGCGGATAAC | Forward primer for construction of pRL100 (Kan promoter) |
| MM710 | CCCATTGGTACCCATATAAATCAGCATC | Reverse primer for construction of pRL100 (Kan promoter) |
| MM744 | CAATAAACCCGTCGACCAATCAAATCC | Forward primer for construction of pRL103 (full ilvIH operon plus promoter) |
| MM745 | CGTCATCTAGAGTTATAAAGCAATTAGGGT | Reverse primer for construction of pRL103 (full ilvIH operon plus promoter) |
| MM588 | CCATGCCGCGTGAATGA | 16S forward Q-PCR primer |
| MM589 | TTCCTCGCTACCGAAAGAACTT | 16S reverse Q-PCR primer |
| MM590 | TGTCGGTCAGCACCAAATGT | ilvI forward Q-PCR primer |
| MM591 | GCGACGAGGTTTTTCAAACG | ilvI reverse Q-PCR primer |
| MM592 | AATTGCTTGAAGCACCGCTATT | lrp forward Q-PCR primer |
| MM593 | CGTCCGGCTTACCTCTGACT | lrp reverse Q-PCR primer |
a
Restriction sites inserted by PCR are underlined.