FIG. 2.
BmpA binds laminin. (A) Purified rBmpA, subjected to SDS-PAGE and stained with Coomassie brilliant blue. (B) Ligand affinity blot analysis, indicating the adherence of laminin to rBmpA. (C) ELISA of varying concentrations of rBmpA binding to purified laminin affixed to wells. Saturated binding was observed, with an apparent Kd of 0.1 μM. (D) ELISA results indicating that neither mammalian fibronectin, collagen I, nor collagen IV detectably bound to immobilized BmpA. Error bars indicate 1 standard deviation from the mean. The asterisk indicates ligand binding to rBmpA that was significantly different from binding to the control protein BSA (P < 0.0001).