TABLE 1.
Primer name | Primer pair sequences (5′ to 3′) |
---|---|
pBmpA | CAC CGG TAA AGG TAG TCT TGG GA |
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT | |
pBmpA-N2 | CAC CCC CGA TAT GAA ATA TGC AAT TAT TG |
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT | |
pBmpA-N3 | CAC CGG TAG AAG CGT TGC AAC TAG |
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT | |
pBmpA-N4 | CAC CGA TGT TGG TAG AGC TTT AAA TAT |
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT | |
pBmpA-I | CAC CCC CGA TAT GAA ATA TGC AAT TAT TG |
TTA AAT TCC TCC AAG GCC TGC AGC | |
pBmpA-C2 | CAC CGG TAA AGG TAG TCT TGG GA |
TTA GGT CAT GCC CAC CAA ATT TGC | |
pBmpB | CAC CTT TAG TAG AAA TGG AAT AGA ATC TAG |
GCA AAA TCC TCT AAA ACA ACA GAA ATG | |
pBmpC | CAC CTT TAA ATC TAA TAA AAA GTC TAT TAA ATC TG |
CCC TTT ACA AAC AAA GCT ATA TTT AAG TAG | |
pBmpD | CAC CTC TAG CTC TGA TGA TGG CAA GTC G |
GAA TTA AAA AGA TTT TTC ACA AAT CAG CTC |
All amplicons were cloned into pET200, and the resultant plasmids were used to produce recombinant proteins.