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. 2009 Aug 24;77(11):4940–4946. doi: 10.1128/IAI.01420-08

TABLE 1.

Oligonucleotides used during this worka

Primer name Primer pair sequences (5′ to 3′)
pBmpA CAC CGG TAA AGG TAG TCT TGG GA
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT
pBmpA-N2 CAC CCC CGA TAT GAA ATA TGC AAT TAT TG
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT
pBmpA-N3 CAC CGG TAG AAG CGT TGC AAC TAG
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT
pBmpA-N4 CAC CGA TGT TGG TAG AGC TTT AAA TAT
CCA TTT CAA TTA TTC AAA CAA AAC CAA TGT
pBmpA-I CAC CCC CGA TAT GAA ATA TGC AAT TAT TG
TTA AAT TCC TCC AAG GCC TGC AGC
pBmpA-C2 CAC CGG TAA AGG TAG TCT TGG GA
TTA GGT CAT GCC CAC CAA ATT TGC
pBmpB CAC CTT TAG TAG AAA TGG AAT AGA ATC TAG
GCA AAA TCC TCT AAA ACA ACA GAA ATG
pBmpC CAC CTT TAA ATC TAA TAA AAA GTC TAT TAA ATC TG
CCC TTT ACA AAC AAA GCT ATA TTT AAG TAG
pBmpD CAC CTC TAG CTC TGA TGA TGG CAA GTC G
GAA TTA AAA AGA TTT TTC ACA AAT CAG CTC
a

All amplicons were cloned into pET200, and the resultant plasmids were used to produce recombinant proteins.