TABLE 2.
Fluorescence microscopy quantification, by direct counts, of the number of differently coated beads associated with CHO-wt and CHO-htpB cells not expressing eHtpB
| Type of bead coating | Mean no. of beads per cell ± SDa |
|||
|---|---|---|---|---|
| CHO-wt cells |
CHO-htpB cellsb |
|||
| 1 h | 3 h | 1 h | 3 h | |
| BSA | 1.6 ± 0.6 | 2 ± 0.9 | 2 ± 0.4 (NS) | 2.1 ± 0.4 (NS) |
| HtpB | 2.7 ± 0.1 | 2.8 ± 1.9 | 2 ± 0.2 | 3 ± 0.8 |
| rHtpB | 2.2 ± 0.4 | 2 ± 0.6 | ND | 3.1 ± 1.0 (NS) |
| GroEL | ND | ND | 3.1 ± 0.2 (NS) | 5 ± 0.8 (NS) |
| In-house GroEL | 2.7 ± 0.7 | 2.4 ± 0.6 | ND | 4.3 ± 0.2 (NS) |
| None | 5.9 ± 1.3 | 5 ± 3.8 | 3.1 ± 0.6 (NS) | 5 ± 1.7 (NS) |
| HtpB + PAbc | 1.5 (n = 1) | 1.7 ± 0.3 | ND | 2.6 ± 1.2 (NS) |
a
The results are the means of two experiments for CHO-wt cells and three experiments for CHO-htpB cells, with 50 to 100 scored cells/experiment.
b
eHtpB was repressed in the presence of 10ng of doxycycline/ml. P values, calculated only for CHO-htpB cells (n = 3) in relation to HtpB-coated beads, indicated no significant differences (NS) at 1 and 3 h.
c
Beads were treated with rHtpB-specific antiserum (PAb) before they were added to the cells.