TABLE 6.
Fluorescence microscopy quantification, by direct counts, of colocalization events between differently coated beads or bacteria and TrOv-labeled lysosomes in CHO-htpB cells not expressing eHtpB
| Type of particlea | Mean % particles colocalized with TrOv ± SD (P) atb: |
|
|---|---|---|
| 1 h postinoculation | 3 h postinoculation | |
| BSA-coated beads | 33 ± 3 (<0.05) | 40 ± 4 (<0.05) |
| HtpB-coated beads | 23 ± 2 | 32 ± 3 |
| GroEL-coated beads | 29 ± 1 (<0.05) | 34 ± 2 (NS) |
| Uncoated beads | 32 ± 1 (<0.05) | 37 ± 1 (NS) |
| HtpB-coated beads + PAb* | 31 ± 4 (<0.05) | 38 ± 2 (NS) |
| GroEL-coated beads + PAb* | 41 ± 1 (<0.05) | 34 ± 3 (NS) |
| L. pneumophila Lp02 | <1 ± 0 (<0.01) | <1 ± 0 (<0.01) |
| Lp02dotB mutant | <1 ± 0 (<0.01) | <1 ± 0 (<0.01) |
a
*, Beads were preincubated with rHtpB-specific rabbit polyclonal antiserum (PAb) before inoculation of the CHO-htpB cells. PAb cross-reacts with GroEL.
b
The results are the means of three experiments, with 100 scored beads or bacteria/experiment. P values, calculated as multiple comparisons, are given in parentheses in relation to HtpB-coated beads. NS, not significant.