FIG. 4.

Kinetics of phagocytic receptor appearance at the onset of monocyte differentiation into DC. Surface expression of CR3, mannose receptor (MR), and DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) was analyzed using freshly isolated monocytes (Mo T0) and monocytes after 12 and 24 h of culture with GM-CSF and IL-4 (Mo 12h and Mo 24h, respectively). The dotted lines indicate data for the appropriate isotype control MAbs. Data from one experiment that is representative of three experiments are shown.