FIG. 2.

Fine specificity of the humoral anti-EF response following A/J immunization. Antibody binding to decapeptides of EF overlapping by 8 aa was measured using a solid-phase ELISA. Shown is the antibody response in sera from rEF/adjuvant-immunized mice on days 14, 28, and 42 and sera from mice immunized with adjuvant alone. Data represent the average of results from three independent immunization/mapping experiments. Mapping within each experiment was performed using sera pooled from 18 to 20 mice. Epitopes were numbered in order of reproducibility and reactivity on day 42, as shown in Table 1. Epitope numbers correspond to epitope numbers in Tables 1 and 2. Horizontal lines indicate extended antigenic regions of epitope 1 as defined on day 42.