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. 2009 Sep 8;77(11):5035–5043. doi: 10.1128/IAI.00695-09

FIG. 2.

FIG. 2.

Bactericidal activity of recombinant Crp4, proCrp4, and mutants with Lys substitutions. Exponentially growing S. aureus (A), L. monocytogenes (B), V. cholerae (C), E. coli ML35 (D), S. Typhimurium 14028S (E), or S. Typhimurium CS022 (F) was exposed to peptides at 37°C in 50 μl of PIPES-TSB buffer for 1 h (see Materials and Methods). Following peptide exposure, the bacteria were plated on TSB-agar plates and incubated overnight at 37°C. Please note the higher peptide concentrations used in assays against S. Typhimurium CS022 (F). Surviving bacteria were counted as CFU/ml at each peptide concentration, and count values below 1 × 103 CFU/ml signify that no colonies were detected. Symbols: proCrp4, ▾; Crp4, •; (R/K)-proCrp4, ▿; and (R/K)-Crp4, ○. (R/K)-Crp4 exhibited reduced activity, particularly against S. Typhimurium strains.