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E. coli ML35 permeabilization by Crp4, proCrp4, and (R/K)-Crp4 at 6 μM. E. coli ML35 cells growing in log phase were exposed to peptides as shown at 37°C in 100 μl PIPES-TSB buffer with 2.5 mM ONPG. ONPG hydrolysis was measured by absorbance at 405 nm for 2 h. Symbols: proCrp4, ▾; Crp4; •; and (R/K)-Crp4, ○. (R/K)-Crp4 displayed delayed E. coli ML35 permeabilization kinetics compared to Crp4.
