TABLE 1.

Binding activities of S. oxalis ATCC 9811 rGAPDH fragments with P. gingivalis rFimA^a

Proteinase(s) used for digestion or type of fragment	S. oralis ATCC 9811 GAPDH fragment		Ka (M−1)
	Amino acid residues	Amino acid sequence
Lysyl endopeptidase	163-216	ALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAK	3.54 × 107
Endoproteinase Asp-N	166-183	---DNFGVVEGLMTTIHAYTG---------------------------------	4.51 × 107
	184-188	---------------------DQMIL----------------------------	0
	189-195	--------------------------DGPHRGG---------------------	0
	196-216	---------------------------------DLRRARAGAANIVPNSTGAAK	0
Trypsin	163-193	ALQDNFGVVEGLMTTIHAYTGDQMILDGPHR-----------------------	3.67 × 107
	202-216	---------------------------------------AGAANIVPNSTGAAK	0
Trypsin and endoproteinase Glu-C	163-172	ALQDNFGVVE--------------------------------------------	0
	173-193	----------GLMTTIHAYTGDQMILDGPHR-----------------------	0
Synthetic peptide	166-183	---DNFGVVEGLMTTIHAYTG---------------------------------	3.84 × 108
	163-179	ALQDNFGVVEGLMTTIH-------------------------------------	3.54 × 106

^aThe binding activities (K_a values) of S. oxalis ATCC 9811 rGAPDH and native GAPDH were determined to be 1.19×10⁷ and 4.34×10⁷ M⁻¹, respectively. Dashes in amino acid sequences represent missing amino acids.