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. 2009 Aug 17;77(11):5163–5169. doi: 10.1128/IAI.00220-09

FIG. 3.

FIG. 3.

Jejunal IELs and mucosal mast cells in the course and after C. parvum infection. Immunostaining for jejunal intraepithelial CD3+ lymphocytes and RMCP-II+ mast cells. (A) Intestinal IELs were counted in each section in 20 well-oriented VCU. Compared to control uninfected rats, the numbers of jejunal IELs/VCU were significantly higher only in NoI-infected rats at day 14 p.i. (i.e., clearance of infection). Values are means ± the SEM (n = 10 in each group). **, P < 0.01 (significantly different from uninfected control rats, as calculated by two-way ANOVA test). (B) Submucosal mast cells were counted in each section on 20 well-oriented VCU. The number of jejunal mast cells was higher in rats infected with NoI-infected than in uninfected control rats from day 36 p.i. to day 120 p.i. Values are means ± the SEM (n = 10 in each group). **, P < 0.01 (significantly different from uninfected control rats as calculated by two-way ANOVA). (C and D) Immunostaining for T IELs with anti-CD3 monoclonal antibody. CD3+ IELs are brown stained. These figures depict representative jejunal sections obtained for control uninfected (C) and NoI-infected (D) rats on day 14 p.i. Original magnification, ×100 (inset, ×1,000). (E and F) Immunostaining for rat RMCP-II+ mast cell protease. RMCP-II+ mucosal mast cells are red stained. These figures depict representative jejunal sections obtained on day 50 p.i. in control uninfected (E) and NoI-infected (F) rats, respectively. Original magnification, ×100 (inset, ×1,000).