FIG. 2.

Mll1 is required for the recruitment of the basal transcription machinery to chromatin. (A) The H3K4me3 level in the absence of Mll1 as determined by ChIP-chip analysis is dramatically decreased at the Il20ra promoter region (black box) but not at the neighboring Pex7 gene (red box). Enrichment profiles for H3K4me3 and total H3 expressed as log₂ ChIP/input. (B) ChIP-qPCR with H3K4me3 antibodies was used to validate the results shown in panel A, using three primer sets spanning the transcription start site. (C and D) The results of ChIP-qPCR with Pol II antibodies (C) or TBP antibodies (D) show that RNA polymerase and TBP recruitment to Il20ra are significantly decreased in the absence of Mll1. Pol II and TBP recruitment on the Pex7 and Gapdh genes, however, did not change in the absence of Mll1. The murine Hba2 gene (mHba2), which is not transcribed in these cells, is used as a negative control. Error bars show standard deviations.